Anti-EEF1A2 antibody (ab235190)
Key features and details
- Chicken polyclonal to EEF1A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-EEF1A2 antibody
See all EEF1A2 primary antibodies -
Description
Chicken polyclonal to EEF1A2 -
Host species
Chicken -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 nuclear extract. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.03% Sodium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Tissue specificity
Brain, heart, and skeletal muscle. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515233 Cow
- Entrez Gene: 1917 Human
- Entrez Gene: 13628 Mouse
- Entrez Gene: 100009189 Rabbit
- Entrez Gene: 24799 Rat
- Omim: 602959 Human
- SwissProt: Q32PH8 Cow
- SwissProt: Q05639 Human
see all -
Alternative names
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
see all
Images
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Anti-EEF1A2 antibody (ab235190) at 5 µg/ml + MCF7 (human breast adenocarcinoma cell line) nuclear extract at 35 µg
Secondary
Goat anti-chicken IgG HRP at 1/5000 dilution
Predicted band size: 50 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for EEF1A2 using ab235190 at a 1/100 dilution (30mins at RT) in immunohistochemistry.
Secondary antibody used was an anti-chicken HRP (1/500, 20mins at RT).
Counterstained with hematoxylin.
HIER using citrate buffer for 20 mins.
Image focused on renal tubular epithelium section.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235190 has not yet been referenced specifically in any publications.