Key features and details
- Chicken polyclonal to EEF1A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgY
Product nameAnti-EEF1A2 antibody
See all EEF1A2 primary antibodies
DescriptionChicken polyclonal to EEF1A2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: MCF7 nuclear extract. IHC-P: Human kidney tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.03% Sodium phosphate, 0.87% Sodium chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab235190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Tissue specificityBrain, heart, and skeletal muscle.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
- Information by UniProt
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
Anti-EEF1A2 antibody (ab235190) at 5 µg/ml + MCF7 (human breast adenocarcinoma cell line) nuclear extract at 35 µg
Goat anti-chicken IgG HRP at 1/5000 dilution
Predicted band size: 50 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for EEF1A2 using ab235190 at a 1/100 dilution (30mins at RT) in immunohistochemistry.
Secondary antibody used was an anti-chicken HRP (1/500, 20mins at RT).
Counterstained with hematoxylin.
HIER using citrate buffer for 20 mins.
Image focused on renal tubular epithelium section.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235190 has not yet been referenced specifically in any publications.