Overview

  • Product name

  • Description

    Chicken polyclonal to EEF1A2
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: MCF7 nuclear extract. IHC-P: Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-EEF1A2 antibody (ab235190) at 5 µg/ml + MCF7 (human breast adenocarcinoma cell line) nuclear extract at 35 µg

    Secondary
    Goat anti-chicken IgG HRP at 1/5000 dilution

    Predicted band size: 50 kDa

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for EEF1A2 using ab235190 at a 1/100 dilution (30mins at RT) in immunohistochemistry.

    Secondary antibody used was an anti-chicken HRP (1/500, 20mins at RT).

    Counterstained with hematoxylin.

    HIER using citrate buffer for 20 mins.

    Image focused on renal tubular epithelium section.

References

ab235190 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235190.
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