Key features and details
- Goat polyclonal to EEF1A2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EEF1A2 antibody
See all EEF1A2 primary antibodies
DescriptionGoat polyclonal to EEF1A2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Human skeletal muscle lysate; MCF7 nuclear extract lysate. IHC-P: Human skeletal muscle tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 50 kDa.|
FunctionThis protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Tissue specificityBrain, heart, and skeletal muscle.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
- Information by UniProt
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
All lanes : Anti-EEF1A2 antibody (ab235235) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear extract lysate
Lane 3 : Mouse adult brain lysate
Lane 4 : Mouse liver lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat IgG (H&L) antibody peroxidase conjugate at 1/5000 dilution
Predicted band size: 50 kDa
Exposure time: 6 seconds
Immunofluorescent analysis of 100% methanol-fixed, 0.3% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling EEF1A2 with ab235235 at 15 µg/ml (Overnight at 4°C), followed by anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green).
Expected localization: nucleoli, cytosol.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling EEF1A2 with ab235235 at 1/100 dilution (30 minutes at RT), followed by a Donkey anti-Goat (HRP) (4 µl/ml, 20 mins at RT). Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Top panel: Human skeletal muscle H&E.
Bottom left panel: ab235235 at 20X magnification. Bottom right panel: ab235235 at 40X magnification.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235235 has not yet been referenced specifically in any publications.