Product nameAnti-EEF1A2 antibody
See all EEF1A2 primary antibodies
DescriptionGoat polyclonal to EEF1A2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Human skeletal muscle lysate; MCF7 nuclear extract lysate. IHC-P: Human skeletal muscle tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 50 kDa.|
FunctionThis protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Tissue specificityBrain, heart, and skeletal muscle.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
- Information by UniProt
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
All lanes : Anti-EEF1A2 antibody (ab235235) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear extract lysate
Lane 3 : Mouse adult brain lysate
Lane 4 : Mouse liver lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat IgG (H&L) antibody peroxidase conjugate at 1/5000 dilution
Predicted band size: 50 kDa
Exposure time: 6 seconds
Immunofluorescent analysis of 100% methanol-fixed, 0.3% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling EEF1A2 with ab235235 at 15 µg/ml (Overnight at 4°C), followed by anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green).
Expected localization: nucleoli, cytosol.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling EEF1A2 with ab235235 at 1/100 dilution (30 minutes at RT), followed by a Donkey anti-Goat (HRP) (4 µl/ml, 20 mins at RT). Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Top panel: Human skeletal muscle H&E.
Bottom left panel: ab235235 at 20X magnification. Bottom right panel: ab235235 at 40X magnification.
ab235235 has not yet been referenced specifically in any publications.