Overview

  • Product name

  • Description

    Goat polyclonal to EEF1A2
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human skeletal muscle lysate; MCF7 nuclear extract lysate. IHC-P: Human skeletal muscle tissue. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 15 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 50 kDa.

Target

Images

  • All lanes : Anti-EEF1A2 antibody (ab235235) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear extract lysate
    Lane 3 : Mouse adult brain lysate
    Lane 4 : Mouse liver lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat IgG (H&L) antibody peroxidase conjugate at 1/5000 dilution

    Predicted band size: 50 kDa


    Exposure time: 6 seconds
  • Immunofluorescent analysis of 100% methanol-fixed, 0.3% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling EEF1A2 with ab235235 at 15 µg/ml (Overnight at 4°C), followed by anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green).

    Expected localization: nucleoli, cytosol.

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling EEF1A2 with ab235235 at 1/100 dilution (30 minutes at RT), followed by a Donkey anti-Goat (HRP) (4 µl/ml, 20 mins at RT). Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

    Top panel: Human skeletal muscle H&E.

    Bottom left panel: ab235235 at 20X magnification. Bottom right panel: ab235235 at 40X magnification.

References

ab235235 has not yet been referenced specifically in any publications.

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