Overview

  • Product name

  • Description

    Rabbit polyclonal to EEF1A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide: VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP, corresponding to a region within internal sequence amino acids 360 - 409 of Human EEF1A2 (NP_001949)

  • Positive control

    • Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

Images

  • Anti-EEF1A2 antibody (ab82912) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Gel concentration 12%
  • All lanes : Anti-EEF1A2 antibody (ab82912) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

References

ab82912 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain, Skeletal Muscle, Kidney)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
Brain, Skeletal Muscle, Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 19 2018

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