Recombinant Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
![Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)](https://www.abcam.com/ps/products/227/ab227824/Images/ab227824-3-anti-eef1a2-antibody-epr-22651-46-western-blot1-human.jpg "Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22651-46] to EEF1A2
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EEF1A2 antibody [EPR22651-46]
See all EEF1A2 primary antibodies -
Description
Rabbit monoclonal [EPR22651-46] to EEF1A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human EEF1A2 aa 200-300. The exact sequence is proprietary.
Database link: Q05639 -
Positive control
- WB: Wild-type HAP1, HEK-293T, Neuro 2a whole cell lysates; Human brain tissue lysate; Mouse and rat brain and heart tissue lysate. IP: HEK-293T whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22651-46 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). | |
| IP | 1/30. |
Target
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Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Tissue specificity
Brain, heart, and skeletal muscle. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1917 Human
- Entrez Gene: 13628 Mouse
- Entrez Gene: 24799 Rat
- Omim: 602959 Human
- SwissProt: Q05639 Human
- SwissProt: P62631 Mouse
- SwissProt: P62632 Rat
- Unigene: 433839 Human
see all -
Alternative names
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
see all
Images
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Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)All lanes : Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : EEF1A2 knockout HAP1 whole cell lysate
Lane 3 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaab227824 was shown to specifically react with EEF1A2 in wild-type HAP1 cells as signal was lost in EEF1A2 knockout cells. Wild-type and EEF1A2 knockout samples were subjected to SDS-PAGE. ab227824 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes a weakly unidentifiable band at around 75kDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3.25 seconds
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![Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)](https://www.abcam.com/ps/products/227/ab227824/Images/ab227824-4-anti-eef1a2-antibody-epr-22651-46-western-blot2-human.jpg)
Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution + Human brain tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 7.75 seconds
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![Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)](https://www.abcam.com/ps/products/227/ab227824/Images/ab227824-2-anti-eef1a2-antibody-epr-22651-46-western-blot-mouse-rat.jpg)
Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)All lanes : Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: 3.25 seconds
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![Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)](https://www.abcam.com/ps/products/227/ab227824/Images/ab227824-1-anti-eef1a2-antibody-epr-22651-46-immunoprecipitation-293t-human.jpg)
Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)EEF1A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab227824 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Ab227824 1/1000 dilution (0.54 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2: ab227824 IP in HEK-293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227824 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
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![Anti-EEF1A2 antibody [EPR22651-46] (ab227824)](https://www.abcam.com/ps/products/227/ab227824/Images/ab227824-5-benefits-of-recombinant-antibodies.png)
Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab227824 has not yet been referenced specifically in any publications.
