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    eef1a2-antibody-epr22651-46-ab227824.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
  • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
  • Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
  • Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22651-46] to EEF1A2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-EEF1A2 antibody [EPR22651-46]
    See all EEF1A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22651-46] to EEF1A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EEF1A2 aa 200-300. The exact sequence is proprietary.
    Database link: Q05639

  • Positive control

    • WB: Wild-type HAP1, HEK-293T, Neuro 2a whole cell lysates; Human brain tissue lysate; Mouse and rat brain and heart tissue lysate. IP: HEK-293T whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 49% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22651-46
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (ab256523)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

Our Abpromise guarantee covers the use of ab227824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
    • Tissue specificity

      Brain, heart, and skeletal muscle.
    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q05639 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1917 Human
      • Entrez Gene: 13628 Mouse
      • Entrez Gene: 24799 Rat
      • Omim: 602959 Human
      • SwissProt: Q05639 Human
      • SwissProt: P62631 Mouse
      • SwissProt: P62632 Rat
      • Unigene: 433839 Human
      • Unigene: 2645 Mouse
      • Unigene: 9764 Rat
      see all
    • Alternative names

      • eEF1A-2 antibody
      • Eef1a2 antibody
      • EEF1AL antibody
      • EF 1 alpha 2 antibody
      • EF-1-alpha-2 antibody
      • EF1A antibody
      • EF1A2_HUMAN antibody
      • Elongation factor 1 A 2 antibody
      • Elongation factor 1 alpha antibody
      • Elongation factor 1 alpha 2 antibody
      • Elongation factor 1-alpha 2 antibody
      • Eukaryotic elongation factor 1 A-2 antibody
      • Eukaryotic translation elongation factor 1 alpha 2 antibody
      • FLJ41696 antibody
      • HS1 antibody
      • OTTHUMP00000031776 antibody
      • Statin like antibody
      • Statin S1 antibody
      • Statin-S1 antibody
      • STN antibody
      • STNL antibody
      see all

    Images

    • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      All lanes : Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : EEF1A2 knockout HAP1 whole cell lysate
      Lane 3 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
      Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      ab227824 was shown to specifically react with EEF1A2 in wild-type HAP1 cells as signal was lost in EEF1A2 knockout cells. Wild-type and EEF1A2 knockout samples were subjected to SDS-PAGE. ab227824 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

      This antibody also recognizes a weakly unidentifiable band at around 75kDa

      Blocking/diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 3.25 seconds

    • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution + Human brain tissue lysate at 20 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      Blocking/diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 7.75 seconds

    • Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      Western blot - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      All lanes : Anti-EEF1A2 antibody [EPR22651-46] (ab227824) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      Blocking/diluting buffer and concentration: 5% NFDM/TBST

      Exposure Time: 3.25 seconds

    • Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

      EEF1A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab227824 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Ab227824 1/1000 dilution (0.54 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

      Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10μg

      Lane 2: ab227824 IP in HEK-293T whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227824 in HEK-293T whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

    • Anti-EEF1A2 antibody [EPR22651-46] (ab227824)
      Anti-EEF1A2 antibody [EPR22651-46] (ab227824)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab227824? Please let us know so that we can cite the reference in this datasheet.

    ab227824 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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