Overview

  • Product name

  • Description

    Rabbit polyclonal to eEF1B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human eEF1B2 (internal sequence). The exact sequence is proprietary.
    Database link: P24534

  • Positive control

    • WB: Mouse brain tissue whole cell lysate; A431, HeLa S3 and NCI-H1299 whole cell lysates. IHC-P: Mouse duodenum tissue; U87 xenograft tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-eEF1B2 antibody (ab228642) at 1/5000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-eEF1B2 antibody (ab228642) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded mouse duodenum tissue stained for eEF1B2 using ab228642 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded U87 xenograft tissue stained for eEF1B2 using ab228642 at 1/250 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for eEF1B2 (green) using ab228642 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: DNA probe (blue).

References

ab228642 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228642.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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