Anti-eEF1B2 antibody (ab228642)
Key features and details
- Rabbit polyclonal to eEF1B2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eEF1B2 antibody
See all eEF1B2 primary antibodies -
Description
Rabbit polyclonal to eEF1B2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human eEF1B2 (internal sequence). The exact sequence is proprietary.
Database link: P24534 -
Positive control
- WB: Mouse brain tissue whole cell lysate; A431, HeLa S3 and NCI-H1299 whole cell lysates. IHC-P: Mouse duodenum tissue; U87 xenograft tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. -
Sequence similarities
Belongs to the EF-1-beta/EF-1-delta family.
Contains 1 GST C-terminal domain. -
Post-translational
modificationsPhosphorylation affects the GDP/GTP exchange rate. - Information by UniProt
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Database links
- Entrez Gene: 395723 Chicken
- Entrez Gene: 1933 Human
- Entrez Gene: 55949 Mouse
- Entrez Gene: 363241 Rat
- Omim: 600655 Human
- SwissProt: Q9YGQ1 Chicken
- SwissProt: P24534 Human
- SwissProt: O70251 Mouse
see all -
Alternative names
- EEF1B antibody
- EEF1B1 antibody
- EEF1B2 antibody
see all
Images
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Anti-eEF1B2 antibody (ab228642) at 1/5000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 24 kDa12% SDS-PAGE gel.
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All lanes : Anti-eEF1B2 antibody (ab228642) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa12% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for eEF1B2 using ab228642 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded U87 xenograft tissue stained for eEF1B2 using ab228642 at 1/250 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for eEF1B2 (green) using ab228642 at 1/200 dilution in ICC/IF.
Nuclear counterstain: DNA probe (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228642 has not yet been referenced specifically in any publications.