Product nameAnti-eEF1B2 antibody
See all eEF1B2 primary antibodies
DescriptionRabbit polyclonal to eEF1B2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Cow, Pig
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate, HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate, MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate and U2OS (Human osteosarcoma cell line) Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).|
|IHC-P||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionEF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
Sequence similaritiesBelongs to the EF-1-beta/EF-1-delta family.
Contains 1 GST C-terminal domain.
modificationsPhosphorylation affects the GDP/GTP exchange rate.
- Information by UniProt
- EEF1B antibody
- EEF1B1 antibody
- EEF1B2 antibody
All lanes : Anti-eEF1B2 antibody (ab37725) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lane 4 :
HEK293 whole cell lysate (ab7902)
Lane 5 :
HepG2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ICC/IF image of ab37725 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37725, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
IHC image of ab37725 staining eEF1B2 in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37725, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Sivan G et al. Mitotic modulation of translation elongation factor 1 leads to hindered tRNA delivery to ribosomes. J Biol Chem 286:27927-35 (2011). WB ; Human . Read more (PubMed: 21665947) »
- Byun HO et al. Cathepsin D and eukaryotic translation elongation factor 1 as promising markers of cellular senescence. Cancer Res 69:4638-47 (2009). WB ; Human . Read more (PubMed: 19487283) »