Product nameAnti-eEF1B2 antibody
See all eEF1B2 primary antibodies
DescriptionRabbit polyclonal to eEF1B2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Chicken, Cow, Pig
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate, HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate, MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate and U2OS (Human osteosarcoma cell line) Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionEF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
Sequence similaritiesBelongs to the EF-1-beta/EF-1-delta family.
Contains 1 GST C-terminal domain.
modificationsPhosphorylation affects the GDP/GTP exchange rate.
- Information by UniProt
- EEF1B antibody
- EEF1B1 antibody
- EEF1B2 antibody
All lanes : Anti-eEF1B2 antibody (ab37957) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lane 4 :
HEK293 whole cell lysate (ab7902)
Lane 5 :
HepG2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ICC/IF image of ab37957 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37957, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab37957 has not yet been referenced specifically in any publications.