Anti-EEF1D antibody (ab153728)
Key features and details
- Rabbit polyclonal to EEF1D
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EEF1D antibody
See all EEF1D primary antibodies -
Description
Rabbit polyclonal to EEF1D -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
A recombinant fragment corresponding to a region within amino acids 1 and 281 of Human EEF1D
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Positive control
- H1299 cell lysate; Mouse brain tissue lysate; A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE). -
Tissue specificity
Isoform 2 is specifically expressed in brain, cerebellum and testis. -
Sequence similarities
Belongs to the EF-1-beta/EF-1-delta family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1936 Human
- Entrez Gene: 66656 Mouse
- Entrez Gene: 300033 Rat
- Omim: 130592 Human
- SwissProt: P29692 Human
- SwissProt: P57776 Mouse
- SwissProt: Q68FR9 Rat
- Unigene: 333388 Human
see all -
Alternative names
- Antigen NY CO 4 antibody
- Antigen NY-CO-4 antibody
- EEF 1D antibody
see all
Images
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Anti-EEF1D antibody (ab153728) at 1/1000 dilution + H1299 cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Anti-EEF1D antibody (ab153728) at 1/3000 dilution + Mouse brain tissue lysate at 50 µg
Predicted band size: 31 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed A431 cells, labeling EEF1D using ab153728 at 1/200 dilution. Right panel costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153728 has not yet been referenced specifically in any publications.