Overview

  • Product name

  • Description

    Rabbit polyclonal to EEF1D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 1 and 281 of Human EEF1D

  • Positive control

    • H1299 cell lysate; Mouse brain tissue lysate; A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab153728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
    Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
  • Tissue specificity

    Isoform 2 is specifically expressed in brain, cerebellum and testis.
  • Sequence similarities

    Belongs to the EF-1-beta/EF-1-delta family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen NY CO 4 antibody
    • Antigen NY-CO-4 antibody
    • EEF 1D antibody
    • EEF1D antibody
    • EF 1 delta antibody
    • EF 1D antibody
    • EF-1-delta antibody
    • EF1D antibody
    • EF1D_HUMAN antibody
    • Elongation factor 1 delta antibody
    • Elongation factor 1-delta antibody
    • eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) antibody
    • Eukaryotic translation elongation factor 1 delta antibody
    • FP1047 antibody
    • Guanine nucleotide exchange protein antibody
    see all

Images

  • Anti-EEF1D antibody (ab153728) at 1/1000 dilution + H1299 cell lysate at 30 µg

    Predicted band size: 31 kDa



    12% SDS PAGE
  • Anti-EEF1D antibody (ab153728) at 1/3000 dilution + Mouse brain tissue lysate at 50 µg

    Predicted band size: 31 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431 cells, labeling EEF1D using ab153728 at 1/200 dilution. Right panel costained with Hoechst 33342.

References

ab153728 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153728.
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