Key features and details
- Rabbit polyclonal to EEF1D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EEF1D antibody
See all EEF1D primary antibodies
DescriptionRabbit polyclonal to EEF1D
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 130 and 180 of Human EEF1D (NP_001951.2)
- HeLa cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 32 kDa.|
|IP||Use at 2-5 - 5 µg/mg of lysate.|
FunctionIsoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
Tissue specificityIsoform 2 is specifically expressed in brain, cerebellum and testis.
Sequence similaritiesBelongs to the EF-1-beta/EF-1-delta family.
- Information by UniProt
- Antigen NY CO 4 antibody
- Antigen NY-CO-4 antibody
- EEF 1D antibody
All lanes : Anti-EEF1D antibody (ab85964) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 3 seconds
1mg HeLa whole cell lysate was immunoprecipitated using 3µg ab85964.
20% of IP was loaded per lane and probed with with ab85964 at 1 µg/ml (lane 1) and with a control IgG (lane 4).
Detection: chemiluminescence with an exposure time of 1 second.
ab85964 has not yet been referenced specifically in any publications.