Key features and details
- Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Product nameAnti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]
See all EEF2/Elongation factor 2 primary antibodies
DescriptionMouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human EEF2/Elongation factor 2.
Database link: NM_001961.3
- HL-60 cell lysate
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
This product was previously labelled as EEF2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol
Concentration information loading...
PurityConcentrated Culture Supernatant
Our Abpromise guarantee covers the use of ab131202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).|
|ELISA||Use at an assay dependent concentration.|
FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
- Information by UniProt
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
All lanes : Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution
Lane 1 : Jurkat Cell lysates
Lane 2 : C6 Cell lysates
Lane 3 : 3T3 Cell lysates
Lane 4 : C0S7 Cell lysates
Lane 5 : HeLa Cell lysates
Predicted band size: 95 kDa
Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites.
Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1:5000).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. This image was produced with antibody produced in ascites.
Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution + HL-60 cell lysate
Predicted band size: 95 kDa
Observed band size: 95 kDa
This image was produced with antibody produced in ascites.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab131202 has not yet been referenced specifically in any publications.