Overview

  • Product name

    Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]
    See all EEF2/Elongation factor 2 primary antibodies
  • Description

    Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human EEF2/Elongation factor 2.
    Database link: NM_001961.3

  • Positive control

    • HL-60 cell lysate
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     This product was previously labelled as EEF2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Concentrated Culture Supernatant
  • Clonality

    Monoclonal
  • Clone number

    4B3-G7-H5
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational
    modifications

    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Images

  • All lanes : Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution

    Lane 1 : Jurkat Cell lysates
    Lane 2 : C6 Cell lysates
    Lane 3 : 3T3 Cell lysates
    Lane 4 : C0S7 Cell lysates
    Lane 5 : HeLa Cell lysates

    Predicted band size: 95 kDa

  • Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites. 

  • Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1:5000).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. This image was produced with antibody produced in ascites. 

  • Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution + HL-60 cell lysate

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    This image was produced with antibody produced in ascites. 

References

ab131202 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Melanie Thelen

Verified customer

Submitted Apr 06 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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