Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
Key features and details
- Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]
See all EEF2/Elongation factor 2 primary antibodies -
Description
Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human EEF2/Elongation factor 2.
Database link: P13639 -
Positive control
- HL-60 cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
4B3-G7-H5 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
Target
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Function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
Post-translational
modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Entrez Gene: 29565 Rat
- Omim: 130610 Human
- SwissProt: P13639 Human
- SwissProt: P58252 Mouse
- SwissProt: P05197 Rat
- Unigene: 515070 Human
see all -
Alternative names
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
Images
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All lanes : Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution
Lane 1 : Jurkat Cell lysates
Lane 2 : C6 Cell lysates
Lane 3 : 3T3 Cell lysates
Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) Cell lysates
Lane 5 : HeLa Cell lysates
Predicted band size: 95 kDa -
Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites.
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Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1:5000).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. This image was produced with antibody produced in ascites.
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Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution + HL-60 cell lysate
Predicted band size: 95 kDa
Observed band size: 95 kDaThis image was produced with antibody produced in ascites.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131202 has not yet been referenced specifically in any publications.