Key features and details
- Rabbit polyclonal to EEF2/Elongation factor 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-EEF2/Elongation factor 2 antibody
See all EEF2/Elongation factor 2 primary antibodies
DescriptionRabbit polyclonal to EEF2/Elongation factor 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Orangutan
Synthetic peptide within Human EEF2/Elongation factor 2 aa 808-858. The exact sequence is proprietary. NP_001952.1
Database link: P13639
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma and mouse teratoma tissue.
This product was previously labelled as EEF2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245412 was affinity purified using an epitope specific to EEF2/Elongation factor 2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 95 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
- Information by UniProt
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
All lanes : Anti-EEF2/Elongation factor 2 antibody (ab245412) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa hole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 1 second
Formalin-fixed, paraffin-embedded human breast carcinoma tissue (left) and mouse teratoma tissue (right) stained for EEF2/Elongation factor 2 using ab245412 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245412 has not yet been referenced specifically in any publications.