Product nameAnti-EEF2/Elongation factor 2 antibody
See all EEF2/Elongation factor 2 primary antibodies
DescriptionRabbit polyclonal to EEF2/Elongation factor 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Orangutan
Synthetic peptide within Human EEF2/Elongation factor 2 aa 808-858. The exact sequence is proprietary. NP_001952.1
Database link: P13639
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma and mouse teratoma tissue.
This product was previously labelled as EEF2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245412 was affinity purified using an epitope specific to EEF2/Elongation factor 2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 95 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
- Information by UniProt
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
All lanes : Anti-EEF2/Elongation factor 2 antibody (ab245412) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa hole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 1 second
Formalin-fixed, paraffin-embedded human breast carcinoma tissue (left) and mouse teratoma tissue (right) stained for EEF2/Elongation factor 2 using ab245412 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
ab245412 has not yet been referenced specifically in any publications.