Overview

  • Product name

    Anti-EEF2/Elongation factor 2 antibody
    See all EEF2/Elongation factor 2 primary antibodies
  • Description

    Rabbit polyclonal to EEF2/Elongation factor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human EEF2/Elongation factor 2 aa 808-858. The exact sequence is proprietary. NP_001952.1
    Database link: P13639

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma and mouse teratoma tissue.
  • General notes

     This product was previously labelled as EEF2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245412 was affinity purified using an epitope specific to EEF2/Elongation factor 2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • Post-translational
      modifications

      Phosphorylation by EF-2 kinase completely inactivates EF-2.
      Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
      ISGylated.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • EEF 2 antibody
      • Eef2 antibody
      • EF-2 antibody
      • EF2 antibody
      • EF2_HUMAN antibody
      • Elongation factor 2 antibody
      • Eukaryotic translation elongation factor 2 antibody
      • Polypeptidyl tRNA translocase antibody
      • SCA26 antibody
      see all

    Images

    • All lanes : Anti-EEF2/Elongation factor 2 antibody (ab245412) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa hole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 95 kDa


      Exposure time: 1 second
    • Formalin-fixed, paraffin-embedded human breast carcinoma tissue (left) and mouse teratoma tissue (right) stained for EEF2/Elongation factor 2 using ab245412 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    References

    ab245412 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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