Overview

  • Product name

    Anti-EEF2/Elongation factor 2 antibody
    See all EEF2/Elongation factor 2 primary antibodies
  • Description

    Rabbit polyclonal to EEF2/Elongation factor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cyt, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human EEF2/Elongation factor 2 aa 800-900 (C terminal).

  • Positive control

    • HEK293 and Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as EEF2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC 1/50 - 1/100.
Flow Cyt 1/20.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/50.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational
    modifications

    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Images

  • Anti-EEF2/Elongation factor 2 antibody (ab40812) at 1/50000 dilution + 10ug HEK293

    Predicted band size: 95 kDa
    Observed band size: 95 kDa

  • ab40812 at a 1/100 dilution staining EEF2/Elongation factor 2 in human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.

References

This product has been referenced in:

  • Nayak T  et al. The Intracellular Cleavage Product of the NG2 Proteoglycan Modulates Translation and Cell-Cycle Kinetics via Effects on mTORC1/FMRP Signaling. Front Cell Neurosci 12:231 (2018). Read more (PubMed: 30131676) »
  • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). Read more (PubMed: 29128334) »
See all 7 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse pancreatic acinar cells)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20 %)
Loading amount
30 µg
Specification
Mouse pancreatic acinar cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up