Key features and details
- Rabbit polyclonal to EEF2K
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EEF2K antibody
See all EEF2K primary antibodies
DescriptionRabbit polyclonal to EEF2K
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Orangutan, Elephant
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human EEF2K, NP_037434.1
- HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 95 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
- Information by UniProt
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
All lanes : Anti-EEF2K antibody (ab85721) at 0.1 µg/ml
Lane 1 : HeLa cells lysate at 50 µg
Lane 2 : HeLa cells lysate at 15 µg
Lane 3 : HeLa cells lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 95 kDa
Additional bands at: 110 kDa, 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EEF2K with ab85721 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human EEF2K by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85721 at 3 ug/mg lysate. Subsequent Western blot detection was performed using 1 ug/ml ab85721. Detection: Chemiluminescence with exposure times of 30 seconds.
ab85721 has not yet been referenced specifically in any publications.