Product nameAnti-EEF2K antibody
See all EEF2K primary antibodies
DescriptionRabbit polyclonal to EEF2K
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-238 of Human EEF2K (NP_037434).
- A431, H1299, HeLa and HepG2 whole cell lysates; A431 cells; Raji cell line
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 82 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
- Information by UniProt
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
All lanes : Anti-EEF2K antibody (ab96685) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa
ab96685, at 1/200 dilution staining EEF2K in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
ab96685 has not yet been referenced specifically in any publications.