Product nameAnti-EEF2K antibody [EP881Y]
See all EEF2K primary antibodies
DescriptionRabbit monoclonal [EP881Y] to EEF2K
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EEF2K aa 700-800 (C terminal). The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab45168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 82 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
- Information by UniProt
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EEF2K knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab45168 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab45168 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab45168 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution + HEK293 cell lysate
Predicted band size: 82 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?