Overview

  • Product name

    Anti-EEF2K (phospho S366) antibody
    See all EEF2K primary antibodies
  • Description

    Rabbit polyclonal to EEF2K (phospho S366)
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366.
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).

  • Positive control

    • Hela cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/300 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
ELISA 1/40000.

Target

  • Function

    Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • C86191 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
    • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
    • Calmodulin dependent protein kinase III antibody
    • cb365 antibody
    • EC 2.7.11.20 antibody
    • eEF 2 kinase antibody
    • eEF 2K antibody
    • eEF-2 kinase antibody
    • eEF-2K antibody
    • Eef2k antibody
    • EEF2K protein antibody
    • EF2K_HUMAN antibody
    • Elongation factor 2 kinase antibody
    • Eukaryotic elongation factor 2 kinase antibody
    • fa04b08 antibody
    • HSU93850 antibody
    • kinase eEF2K antibody
    • MGC45041 antibody
    • SMEF2K antibody
    • wu:fa04b08 antibody
    • zgc:100973 antibody
    see all

Images

  • All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution

    Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
    Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.

    Predicted band size: 95 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)

References

ab51227 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse brain tissue)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
25 µg
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 5% · Temperature: 23°C

Tehila Beiser

Verified customer

Submitted Jun 18 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Specification
colon
Blocking step
background sniper as blocking agent for 8 minute(s) · Concentration: 100%
Fixative
formalin

Abcam user community

Verified customer

Submitted Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up