Product nameAnti-EEF2K (phospho S366) antibody
See all EEF2K primary antibodies
DescriptionRabbit polyclonal to EEF2K (phospho S366)
SpecificityThis antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366.
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).
- Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab51227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/300 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).|
FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
- Information by UniProt
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)
ab51227 has not yet been referenced specifically in any publications.