Anti-EEF2K (phospho S366) antibody (ab51227)
Key features and details
- Rabbit polyclonal to EEF2K (phospho S366)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2K (phospho S366) antibody
See all EEF2K primary antibodies -
Description
Rabbit polyclonal to EEF2K (phospho S366) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).
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Positive control
- Hela cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab51227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. | |
| WB | 1/300 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). |
Target
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Function
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
Sequence similarities
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: O08796 Mouse
- SwissProt: P70531 Rat
- Unigene: 498892 Human
see all -
Alternative names
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
Images
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Western blot - Anti-EEF2K (phospho S366) antibody (ab51227)All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S366) antibody (ab51227)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)
Protocols
Datasheets and documents
References (0)
ab51227 has not yet been referenced specifically in any publications.
