Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse brain tissue)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
25 µg
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Diluent: 5% BSA in TBST
Dilution
1/300
Detection
Detection method
BioRad ChemiDoc™ XRS+ Imaging system
Exposure
1 minute(s) and 51 second(s)
Additional data
Additional Notes
Please find attached a 111s exposure, were no blots around 100kDa could be detected.
Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. This antibody has not been tested with this WB / mouse before, and it is possible that it may not be suitable for use.
Tehila Beiser
Verified customer
Submitted Jun 18 2019