Key features and details
- Rabbit polyclonal to EEFSEC
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-EEFSEC antibody
See all EEFSEC primary antibodies
DescriptionRabbit polyclonal to EEFSEC
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human EEFSEC (internal sequence). The exact sequence is proprietary.
Database link: P57772
- ICC/IF: A431 cells. IHC-P: Mouse stomach tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
RelevanceEEFSEC is a translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
Cellular localizationCytoplasmic and Nuclear
- EFSEC antibody
- Elongation factor for selenoprotein translation antibody
- Elongation factor sec antibody
A431 (human epidermoid carcinoma cell line) cells stained for EEFSEC (green) using ab229160 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS at RT for 15 minutes.
Nucelar counterstain: Hoechst 33342 (blue).
Paraffin-embedded mouse stomach tissue stained for EEFSEC with ab229160 at a 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229160 has not yet been referenced specifically in any publications.