Key features and details
- Rabbit polyclonal to EEN
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EEN antibody
See all EEN primary antibodies
DescriptionRabbit polyclonal to EEN
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- WB: HT-29 and HEK-293T whole cell lysates. IHC-P: Human placenta tissue.
This product was previously labelled as SH3 containing Grb 2 like 1 protein
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 35,36,41 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionImplicated in endocytosis. May recruit other proteins to membranes with high curvature.
Tissue specificityUbiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
Involvement in diseaseNote=In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene.
Sequence similaritiesBelongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
Cellular localizationCytoplasm. Early endosome membrane. Associated with postsynaptic endosomes in hippocampal neurons.
- Information by UniProt
- CNSA1 antibody
- EEN antibody
- EEN fusion partner of MLL antibody
All lanes : Anti-EEN antibody (ab230036) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 35,36,41 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Paraffin-embedded human placenta tissue stained for EEN using ab230036 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230036 has not yet been referenced specifically in any publications.