Overview

  • Product name

  • Description

    Rabbit polyclonal to EEN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human EEN aa 225-275.
    Database link: NP_003016.1

  • Positive control

    • HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SH3 containing Grb 2 like 1 protein

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Implicated in endocytosis. May recruit other proteins to membranes with high curvature.
  • Tissue specificity

    Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
  • Involvement in disease

    Note=In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene.
  • Sequence similarities

    Belongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Domain

    An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
  • Cellular localization

    Cytoplasm. Early endosome membrane. Associated with postsynaptic endosomes in hippocampal neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • CNSA1 antibody
    • EEN antibody
    • EEN fusion partner of MLL antibody
    • Endophilin-2 antibody
    • Endophilin-A2 antibody
    • Extra 11 19 leukemia fusion gene antibody
    • Extra eleven-nineteen leukemia fusion gene protein antibody
    • Fusion partner of MLL antibody
    • MGC111371 antibody
    • SH3 containing Grb 2 like 1 protein antibody
    • SH3 containing protein EEN antibody
    • SH3 domain GRB2 like 1 antibody
    • SH3 domain protein 2B antibody
    • SH3 domain-containing GRB2-like protein 1 antibody
    • SH3D2B antibody
    • SH3G1_HUMAN antibody
    • Sh3gl1 antibody
    • SH3P8 antibody
    see all

Images

  • All lanes : Anti-EEN antibody (ab86605) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 41 kDa


    Exposure time: 30 seconds
  • Detection of EEN by Western Blot of Immunprecipitate.
    ab86605 at 1µg/ml staining EEN in HeLa whole cell lysate immunoprecipitated using ab86605 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab86605 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86605.
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