Key features and details
- Rabbit polyclonal to EEN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EEN antibody
See all EEN primary antibodies
DescriptionRabbit polyclonal to EEN
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human EEN aa 225-275.
Database link: NP_003016.1
- HeLa whole cell lysate.
This product was previously labelled as SH3 containing Grb 2 like 1 protein
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionImplicated in endocytosis. May recruit other proteins to membranes with high curvature.
Tissue specificityUbiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
Involvement in diseaseNote=In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene.
Sequence similaritiesBelongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
Cellular localizationCytoplasm. Early endosome membrane. Associated with postsynaptic endosomes in hippocampal neurons.
- Information by UniProt
- CNSA1 antibody
- EEN antibody
- EEN fusion partner of MLL antibody
All lanes : Anti-EEN antibody (ab86605) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 41 kDa
Exposure time: 30 seconds
Detection of EEN by Western Blot of Immunprecipitate.
ab86605 at 1µg/ml staining EEN in HeLa whole cell lysate immunoprecipitated using ab86605 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab86605 has not yet been referenced specifically in any publications.