Key features and details
- Rabbit polyclonal to EEN
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EEN antibody
See all EEN primary antibodies
DescriptionRabbit polyclonal to EEN
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human EEN aa 152-342. (AAH01270)
- 293T, A431, H1299, HeLa, and Molt-4 cell lysates. HepG2 and Raji whole cell lysate. HeLa cells.
This product was previously labelled as SH3 containing Grb 2 like 1 protein
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionImplicated in endocytosis. May recruit other proteins to membranes with high curvature.
Tissue specificityUbiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
Involvement in diseaseNote=In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene.
Sequence similaritiesBelongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
Cellular localizationCytoplasm. Early endosome membrane. Associated with postsynaptic endosomes in hippocampal neurons.
- Information by UniProt
- CNSA1 antibody
- EEN antibody
- EEN fusion partner of MLL antibody
All lanes : Anti-EEN antibody (ab97336) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEN antibody (ab97336) at 1/200 dilution.
ab97336 has not yet been referenced specifically in any publications.