Product nameAnti-EF-Ts antibody [EPR12034]
See all EF-Ts primary antibodies
DescriptionRabbit monoclonal [EPR12034] to EF-Ts
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EF-Ts aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P43897
- HeLa, Jurkat, 293T and HepG2 cell lysates. HeLa cells. Immunoprecipitation pellet from 293T cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab173528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 35 kDa.|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionAssociates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
Tissue specificityExpressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.
Involvement in diseaseDefects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3) [MIM:610505]. Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes.
Sequence similaritiesBelongs to the EF-Ts family.
- Information by UniProt
- COXPD 3 antibody
- COXPD3 antibody
- EF TS antibody
All lanes : Anti-EF-Ts antibody [EPR12034] (ab173528) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : 293T cell lysates
Lane 4 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Immunofluorescence analysis of HeLa cells labeling EF-Ts with ab173528 at a 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from 293T cell lysate: ab173528 at a 1/10 dilution was used to immunoprecipitated EF-Ts.
ab173528 has not yet been referenced specifically in any publications.