Recombinant Anti-EF-Ts antibody [EPR12034] (ab173528)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12034] to EF-Ts
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EF-Ts antibody [EPR12034]
See all EF-Ts primary antibodies -
Description
Rabbit monoclonal [EPR12034] to EF-Ts -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human EF-Ts aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P43897 -
Positive control
- HeLa, Jurkat, 293T and HepG2 cell lysates. HeLa cells. Immunoprecipitation pellet from 293T cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12034 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
|
ICC/IF |
1/50 - 1/100.
|
|
IP |
1/10 - 1/100.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
Target
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Function
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. -
Tissue specificity
Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney. -
Involvement in disease
Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3) [MIM:610505]. Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes. -
Sequence similarities
Belongs to the EF-Ts family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 10102 Human
- Entrez Gene: 66399 Mouse
- Entrez Gene: 679068 Rat
- Omim: 604723 Human
- SwissProt: P43897 Human
- SwissProt: Q9CZR8 Mouse
- SwissProt: Q9QYU2 Rat
- Unigene: 632704 Human
see all -
Alternative names
- COXPD 3 antibody
- COXPD3 antibody
- EF TS antibody
see all
Images
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All lanes : Anti-EF-Ts antibody [EPR12034] (ab173528) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
Immunofluorescence analysis of HeLa cells labeling EF-Ts with ab173528 at a 1/50 dilution.
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate: ab173528 at a 1/10 dilution was used to immunoprecipitated EF-Ts.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab173528 has been referenced in 1 publication.
- Yokokawa T et al. Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Physiol Rep 6:e13893 (2018). PubMed: 30369085