Recombinant Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13742] to EFCAB4A - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free
See all EFCAB4A primary antibodies -
Description
Rabbit monoclonal [EPR13742] to EFCAB4A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250821 is the carrier-free version of ab185223. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250821 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13742 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Plays a role in store-operated Ca(2+) entry (SOCE). -
Sequence similarities
Belongs to the EFCAB4 family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 283229 Human
- Entrez Gene: 213573 Mouse
- Entrez Gene: 309112 Rat
- Omim: 614177 Human
- SwissProt: Q8N4Y2 Human
- SwissProt: Q80ZJ8 Mouse
- SwissProt: B0BNK9 Rat
- Unigene: 660936 Human
see all -
Alternative names
- Calcium release-activated calcium channel regulator 2B antibody
- CRAC channel regulator 2B antibody
- CRACR2B antibody
see all
Images
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All lanes : Anti-EFCAB4A antibody [EPR13742] (ab185223) at 1/1000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : LnCaP cell lysate
Lane 3 : SW480 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaThis data was developed using ab185223, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab250821 has not yet been referenced specifically in any publications.