RecombinantRabMAb

Recombinant Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)

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Western blot - Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)
  • Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13742] to EFCAB4A - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free
    See all EFCAB4A primary antibodies

  • Description

    Rabbit monoclonal [EPR13742] to EFCAB4A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250821 is the carrier-free version of ab185223. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250821 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

Images

  • Western blot - Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)
    Western blot - Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)
    All lanes : Anti-EFCAB4A antibody [EPR13742] (ab185223) at 1/1000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab185223, the same antibody clone in a different buffer formulation.

     

  • Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)
    Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (ab250821)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250821? Please let us know so that we can cite the reference in this datasheet.

ab250821 has not yet been referenced specifically in any publications.

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