Anti-EFCAB7 antibody (ab229979)
Key features and details
- Rabbit polyclonal to EFCAB7
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EFCAB7 antibody -
Description
Rabbit polyclonal to EFCAB7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human EFCAB7 aa 1-200.
Database link: A8K855 -
Positive control
- WB: HEK-293 whole cell lysate; Mouse skeletal muscle lysate. IHC-P: Human liver cancer and kidney tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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WB |
1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
WB
1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
Target
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Sequence similarities
Contains 3 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 84455 Human
- Entrez Gene: 230500 Mouse
- SwissProt: A8K855 Human
- SwissProt: Q8VDY4 Mouse
- Unigene: 652324 Human
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Alternative names
- EF hand calcium binding domain 7 antibody
- EF-hand calcium-binding domain-containing protein 7 antibody
- Efcab7 antibody
see all
Images
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All lanes : Anti-EFCAB7 antibody (ab229979) at 1/2000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa -
Paraffin-embedded human liver cancer tissue stained for EFCAB7 using ab229979 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for EFCAB7 using ab229979 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for EFCAB7 (green) using ab229979 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229979 has not yet been referenced specifically in any publications.