Key features and details
- Rabbit polyclonal to EFEMP1/Fibulin-3
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EFEMP1/Fibulin-3 antibody
See all EFEMP1/Fibulin-3 primary antibodies
DescriptionRabbit polyclonal to EFEMP1/Fibulin-3
Specificityab106429 is predicted not to cross-react with other Fibulin proteins.
Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human EFEMP1/Fibulin-3 (C terminal).
Database link: Q12805
- HeLa cell lysate.
Antibodies should not be exposed to prolonged high temperatures.
This product was previously labelled as EFEMP1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.|
|ICC||Use a concentration of 20 µg/ml.|
FunctionBinds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Tissue specificityIn the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina.
Involvement in diseaseDefects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD) [MIM:126600]; also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium.
Sequence similaritiesBelongs to the fibulin family.
Contains 6 EGF-like domains.
Cellular localizationSecreted > extracellular space. Secreted > extracellular space > extracellular matrix. Localizes to the lamina propria underneath the olfactory epithelium.
- Information by UniProt
- DHRD antibody
- Doyne honeycomb retinal degeneration antibody
- DRAD antibody
Lane 1 : Anti-EFEMP1/Fibulin-3 antibody (ab106429) at 0.5 µg/ml
Lane 2 : Anti-EFEMP1/Fibulin-3 antibody (ab106429) at 1 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 55 kDa
Immunohistochemical analysis of HeLa cells labelling EFEMP1/Fibulin-3 with ab106429 at 20 µg/ml.
Immunofluorescence analysis of Hela cells labelling Fibulin 3 with ab106429 at 20 ug/ml.
ab106429 has been referenced in 2 publications.
- Takahashi R et al. Defining Transcriptional Signatures of Human Hair Follicle Cell States. J Invest Dermatol 140:764-773.e4 (2020). PubMed: 31676413
- Shao L et al. Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes. Reprod Fertil Dev N/A:N/A (2015). PubMed: 25989843