Overview

  • Product name

    Anti-EFEMP1/Fibulin-3 antibody
    See all EFEMP1/Fibulin-3 primary antibodies
  • Description

    Rabbit polyclonal to EFEMP1/Fibulin-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human EFEMP1/Fibulin-3 (internal sequence). The exact sequence is proprietary.
    Database link: Q12805

  • Positive control

    • WB: Raji whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
  • General notes

     This product was previously labelled as EFEMP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
  • Tissue specificity

    In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina.
  • Involvement in disease

    Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD) [MIM:126600]; also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium.
  • Sequence similarities

    Belongs to the fibulin family.
    Contains 6 EGF-like domains.
  • Cellular localization

    Secreted > extracellular space. Secreted > extracellular space > extracellular matrix. Localizes to the lamina propria underneath the olfactory epithelium.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHRD antibody
    • Doyne honeycomb retinal degeneration antibody
    • DRAD antibody
    • EFEMP 1 antibody
    • EFEMP1 antibody
    • EGF containing fibulin like extracellular matrix protein 1 antibody
    • EGF containing fibulin like extracellular matrix protein 1 precursor antibody
    • EGF-containing fibulin-like extracellular matrix protein 1 antibody
    • Epidermal growth factor containing fibulin like extracellular matrix protein 1 antibody
    • Extracellular protein S1 5 antibody
    • Extracellular protein S1-5 antibody
    • FBLN 3 antibody
    • FBLN3 antibody
    • FBLN3_HUMAN antibody
    • FBNL antibody
    • FIBL 3 antibody
    • FIBL-3 antibody
    • FIBL3 antibody
    • Fibrillin like antibody
    • Fibrillin like protein antibody
    • Fibrillin-like protein antibody
    • FIBULIN 3 antibody
    • Fibulin-3 antibody
    • Fibulin3 antibody
    • FLJ35535 antibody
    • Heat shock 70 KD protein 1 antibody
    • MGC111353 antibody
    • MLVT antibody
    • MTLV antibody
    • S1 5 antibody
    • T16 protein antibody
    see all

Images

  • Anti-EFEMP1/Fibulin-3 antibody (ab228797) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    10% SDS-PAGE

  • Paraffin-embedded human colon carcinoma tissue stained for EFEMP1/Fibulin-3 using ab228797 at 1/250 dilution in immunohistochemical analysis.

  • A431 (human epidermoid carcinoma cell line) cells stained for EFEMP1/Fibulin-3 (green) using ab228797 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).

References

ab228797 has not yet been referenced specifically in any publications.

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