Overview

  • Product name

    Anti-EFHD2/SWS1 antibody [78.3_6D12]
    See all EFHD2/SWS1 primary antibodies
  • Description

    Mouse monoclonal [78.3_6D12] to EFHD2/SWS1
  • Host species

    Mouse
  • Specificity

    ab128063 specificity has been determined by protein microarray.
  • Tested applications

    Suitable for: WB, IP, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human EFHD2/SWS1.

  • Positive control

    • Transfected HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab128063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
IP Use a concentration of 4 µg/ml.
ELISA Use at an assay dependent dilution.

Target

  • Function

    May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
  • Tissue specificity

    Found in lymphocytes; preferentially expressed in CD8+ cells.
  • Sequence similarities

    Contains 2 EF-hand domains.
  • Cellular localization

    Membrane raft. In immature B-cell line WEHI231.
  • Information by UniProt
  • Database links

  • Alternative names

    • EF hand domain containing protein 2 antibody
    • EF hand domain family, member D2 antibody
    • EF-hand domain-containing protein D2 antibody
    • EFHD 2 antibody
    • EFHD2 antibody
    • EFHD2_HUMAN antibody
    • RP3-467K16.3 antibody
    • Swiprosin 1 antibody
    • Swiprosin-1 antibody
    • SWS1 antibody
    see all

Images

  • 2 µg of ab128063 was added to aliquots of cleared HeLa cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
    Lane 1: Cell Lysate control
    Lane 2: Cell lysate with ab128063
    Lane 3: Cell lysate without ab128063
    Lane 4: V5 tag control
  • Lanes 1-3 : Anti-EFHD2/SWS1 antibody [78.3_6D12] (ab128063)
    Lanes 4-6 : V5 antibody control

    Lanes 1 & 5 : Non-Transfected HeLa cell lysate
    Lanes 2 & 4 : Transfected HeLa cell lysate
    Lanes 3 & 6 : SH-SY5Y cell lysate

    Predicted band size: 26 kDa

References

ab128063 has not yet been referenced specifically in any publications.

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