• Product name

  • Description

    Rabbit polyclonal to EFTUD2
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residues 1 and 50 of EFTUD2. (NP_004238.2)

  • Positive control

    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab72456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 109 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
  • Involvement in disease

    Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM) [MIM:610536]. A rare syndrome characterized by progressive microcephaly, midface and malar hypoplasia, micrognathia, microtia, dysplastic ears, preauricular skin tags, significant developmental delay, and speech delay. Many patients have major sequelae, including choanal atresia that results in respiratory difficulties, conductive hearing loss, and cleft palate.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 116 kDa antibody
    • 116 kDa U5 small nuclear ribonucleoprotein component antibody
    • EFTUD2 antibody
    • Elongation factor Tu GTP binding domain containing 2 antibody
    • Elongation factor Tu GTP-binding domain-containing protein 2 antibody
    • hSNU114 antibody
    • MFDGA antibody
    • MFDM antibody
    • SNRNP116 antibody
    • Snrp116 antibody
    • Snu114 antibody
    • SNU114 homolog antibody
    • U5 116KD antibody
    • U5 small nuclear ribonucleoprotein component antibody
    • U5 snRNP specific protein, 116 kD antibody
    • U5 snRNP specific protein, 116 kDa antibody
    • U5 snRNP-specific protein antibody
    • U5-116 kDa antibody
    • U5-116KD antibody
    • U5S1_HUMAN antibody
    see all


  • All lanes : Anti-EFTUD2 antibody (ab72456) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 109 kDa
    Observed band size: 117 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling EFTUD2 with ab72456 at 1/200 (1µg/ml). Detection: DAB.
  • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72456 used for IP at 3 µg/mg lysate, and at 0.1 µg/ml for subsequent WB.
    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • IHC image of ab72456 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab110240 staining EFTUD2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% donkey serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000 in PBS) for 1.5 hours at 25°C. A Cy3®-conjugated sheep anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody. DNA was stained with TO-PRO®-3.

    See Abreview


This product has been referenced in:

  • Cloutier P  et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . Read more (PubMed: 28561026) »
See 1 Publication for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - Triton 0.3%
Blocking step
Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jul 02 2012

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