Overview

  • Product name

    Anti-EFTUD2 antibody [EPR16055(B)]
    See all EFTUD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR16055(B)] to EFTUD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EFTUD2 aa 50-150. The exact sequence is proprietary.
    Database link: Q15029

  • Positive control

    • HeLa, Jurkat, 293. U87-MG, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Human colon tissue and Human ovarian carcinoma tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16055(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).

Target

  • Function

    Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
  • Involvement in disease

    Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM) [MIM:610536]. A rare syndrome characterized by progressive microcephaly, midface and malar hypoplasia, micrognathia, microtia, dysplastic ears, preauricular skin tags, significant developmental delay, and speech delay. Many patients have major sequelae, including choanal atresia that results in respiratory difficulties, conductive hearing loss, and cleft palate.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 116 kDa antibody
    • 116 kDa U5 small nuclear ribonucleoprotein component antibody
    • EFTUD2 antibody
    • Elongation factor Tu GTP binding domain containing 2 antibody
    • Elongation factor Tu GTP-binding domain-containing protein 2 antibody
    • hSNU114 antibody
    • MFDGA antibody
    • MFDM antibody
    • SNRNP116 antibody
    • Snrp116 antibody
    • Snu114 antibody
    • SNU114 homolog antibody
    • U5 116KD antibody
    • U5 small nuclear ribonucleoprotein component antibody
    • U5 snRNP specific protein, 116 kD antibody
    • U5 snRNP specific protein, 116 kDa antibody
    • U5 snRNP-specific protein antibody
    • U5-116 kDa antibody
    • U5-116KD antibody
    • U5S1_HUMAN antibody
    see all

Images

  • All lanes : Anti-EFTUD2 antibody [EPR16055(B)] (ab188327) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EFTUD2 with ab188327 at 1/250 dilution followed by Goat anti rabbit IgG (Dylight 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • All lanes : Anti-EFTUD2 antibody [EPR16055(B)] (ab188327) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling EFTUD2 with ab188327 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EFTUD2 with ab188327 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab188327 has not yet been referenced specifically in any publications.

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