Product nameAnti-Eg5 antibody
See all Eg5 primary antibodies
DescriptionRabbit polyclonal to Eg5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide aa 925-975. The exact sequence is proprietary.
Database link: P52732
- WB: HeLa whole cell lysate; IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMotor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Involvement in diseaseDefects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
Sequence similaritiesBelongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.
modificationsPhosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > spindle pole.
- Information by UniProt
- EG5 antibody
- HKSP antibody
- KIF11 antibody
All lanes : Anti-Eg5 antibody (ab264176) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Exposure time: 3 seconds
Eg5 was immunoprecipitated from 1mg of HeLa cells (20% of IP loaded) using ab264176 at 1 µg/ml. Western blot was performed on the immunprecipitate using ab264176 at 3 μg/mg lysate.
Lane 1: ab264176 in HeLa whole cell lysate
Lane 2: Control IgG in HeLa whole cell lysate
Detected with chemilimuniscence with exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264176 has not yet been referenced specifically in any publications.