Product nameAnti-Eg5 antibody - C-terminal
See all Eg5 primary antibodies
DescriptionRabbit polyclonal to Eg5 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within C terminal amino acids 752-1033 of Human Eg5 (P52732).
- HeLaS3 and H1299 whole cell lysate; A431 cells; Cal27 xenograft tissue; 293T, A431, HeLaS3, HepG2, Molt-4 and Raji cell lines.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 119 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionMotor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Involvement in diseaseDefects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
Sequence similaritiesBelongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.
modificationsPhosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > spindle pole.
- Information by UniProt
- EG5 antibody
- HKSP antibody
- KIF11 antibody
All lanes : Anti-Eg5 antibody - C-terminal (ab137535) at 1/5000 dilution
Lane 1 : H1299 cell lysate
Lane 2 : HeLaS3 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 119 kDa
Immunofluorescence analysis of paraformaldehyde-fixed A431 labelling Eg5 with ab137535 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labelling Eg5 with ab137535 at 1/500 dilution.
This product has been referenced in:
- Rao SR et al. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics 15:2537-53 (2016). Read more (PubMed: 27174698) »
- Wu JM et al. Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules. Proc Natl Acad Sci U S A 110:E1779-87 (2013). Read more (PubMed: 23610398) »