Overview

  • Product name

    Anti-Eg5 antibody - C-terminal
    See all Eg5 primary antibodies
  • Description

    Rabbit polyclonal to Eg5 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 752-1033 of Human Eg5 (P52732).

  • Positive control

    • HeLaS3 and H1299 whole cell lysate; A431 cells; Cal27 xenograft tissue; 293T, A431, HeLaS3, HepG2, Molt-4 and Raji cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab137535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 119 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
  • Involvement in disease

    Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
  • Sequence similarities

    Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications

    Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt
  • Database links

  • Alternative names

    • EG5 antibody
    • HKSP antibody
    • KIF11 antibody
    • KIF11_HUMAN antibody
    • Kinesin family member 11 antibody
    • Kinesin like protein 1 antibody
    • Kinesin-like protein 1 antibody
    • Kinesin-like protein KIF11 antibody
    • Kinesin-like spindle protein HKSP antibody
    • Kinesin-related motor protein Eg5 antibody
    • KNSL1 antibody
    • MCLMR antibody
    • Thyroid receptor-interacting protein 5 antibody
    • TR-interacting protein 5 antibody
    • TRIP-5 antibody
    • TRIP5 antibody
    see all

Images

  • All lanes : Anti-Eg5 antibody - C-terminal (ab137535) at 1/5000 dilution

    Lane 1 : H1299 cell lysate
    Lane 2 : HeLaS3 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 119 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed A431 labelling Eg5 with ab137535 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labelling Eg5 with ab137535 at 1/500 dilution.

References

This product has been referenced in:

  • Rao SR  et al. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics 15:2537-53 (2016). Read more (PubMed: 27174698) »
  • Wu JM  et al. Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules. Proc Natl Acad Sci U S A 110:E1779-87 (2013). Read more (PubMed: 23610398) »
See all 2 Publications for this product

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