Product nameAnti-Eg5 antibody [EPR12280-76]
See all Eg5 primary antibodies
DescriptionRabbit monoclonal [EPR12280-76] to Eg5
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Eg5 aa 900 to the C-terminus. The exact sequence is proprietary.
Database link: P52732
- Raji, HeLa and K562 cell lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 119 kDa.|
FunctionMotor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Involvement in diseaseDefects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
Sequence similaritiesBelongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.
modificationsPhosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > spindle pole.
- Information by UniProt
- EG5 antibody
- HKSP antibody
- KIF11 antibody
Immunofluorescence analysis of -20℃ acetone fixed HeLa cells labeling Eg5 with ab181981 at 1/100 dilution, followed by goat anti rabbit IgG (Dylight 555) at a 1/1000 dilution. Counter stained with Dapi.
All lanes : Anti-Eg5 antibody [EPR12280-76] (ab181981) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 119 kDa
ab181981 has been referenced in 2 publications.
- Tan Z et al. Environmental stresses induce karyotypic instability in colorectal cancer cells. Mol Biol Cell 30:42-55 (2019). PubMed: 30379607
- Su KC et al. CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses. Mol Biol Cell 29:2370-2377 (2018). PubMed: 30091644