Product nameAnti-Eg5 antibody [EPR12280]
See all Eg5 primary antibodies
DescriptionRabbit monoclonal [EPR12280] to Eg5
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant fragment within Human Eg5. The exact sequence is proprietary.
Database link: P52732
- HeLa cells, Raji, HeLa, K-562 and Jurkat cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 119 kDa.|
FunctionMotor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Involvement in diseaseDefects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
Sequence similaritiesBelongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.
modificationsPhosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > spindle pole.
- Information by UniProt
- EG5 antibody
- HKSP antibody
- KIF11 antibody
All lanes : Anti-Eg5 antibody [EPR12280] (ab171963) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 119 kDa
Immunofluorescence analysis of HeLa cells, labeling Eg5 using ab171963 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate, labeling Eg5 using ab171963 at a 1/10 dilution, and an IgG control.
ab171963 has not yet been referenced specifically in any publications.