Recombinant Anti-Eg5 antibody [EPR12280] (ab171963)
![Western blot - Anti-Eg5 antibody [EPR12280] (ab171963)](https://www.abcam.com/ps/products/171/ab171963/Images/ab171963-172656-ab171963wb.jpg "Western blot - Anti-Eg5 antibody [EPR12280] (ab171963)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12280] to Eg5
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-Eg5 antibody [EPR12280]
See all Eg5 primary antibodies -
Description
Rabbit monoclonal [EPR12280] to Eg5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment within Human Eg5. The exact sequence is proprietary.
Database link: P52732 -
Positive control
- HeLa cells, Raji, HeLa, K-562 and Jurkat cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12280 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab171963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 119 kDa. |
Target
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Function
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. -
Involvement in disease
Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes. -
Sequence similarities
Belongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain. -
Post-translational
modificationsPhosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. - Information by UniProt
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Database links
- Entrez Gene: 3832 Human
- Omim: 148760 Human
- SwissProt: P52732 Human
- Unigene: 8878 Human
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Alternative names
- EG5 antibody
- HKSP antibody
- KIF11 antibody
see all
Images
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Western blot - Anti-Eg5 antibody [EPR12280] (ab171963)All lanes : Anti-Eg5 antibody [EPR12280] (ab171963) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 119 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR12280] (ab171963)](https://www.abcam.com/ps/products/171/ab171963/Images/ab171963-172658-ab171963if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR12280] (ab171963)Immunofluorescence analysis of HeLa cells, labeling Eg5 using ab171963 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-Eg5 antibody [EPR12280] (ab171963)](https://www.abcam.com/ps/products/171/ab171963/Images/ab171963-172657-ab171963ip.jpg)
Immunoprecipitation - Anti-Eg5 antibody [EPR12280] (ab171963)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate, labeling Eg5 using ab171963 at a 1/10 dilution, and an IgG control.
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![Anti-Eg5 antibody [EPR12280] (ab171963)](https://www.abcam.com/ps/products/171/ab171963/Images/ab171963-4-benefits-of-recombinant-antibodies.png)
Anti-Eg5 antibody [EPR12280] (ab171963)
Protocols
Datasheets and documents
References (0)
ab171963 has not yet been referenced specifically in any publications.
