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  1. Link

    eg5-antibody-mabcam-51976-ab51976.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Kinesin-like
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Anti-Eg5 antibody [mAbcam 51976] (ab51976)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (6)

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Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
  • Western blot - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
  • Immunoprecipitation - Anti-Eg5 antibody [mAbcam 51976] (ab51976)

Key features and details

  • Mouse monoclonal [mAbcam 51976] to Eg5
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

You may also be interested in

Protein
Product image
Recombinant Human Eg5 protein (ab152491)
Array
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Human Chemokine Antibody Array - Membrane (38 targets) (ab169812)
Primary
Product image
Alexa Fluor® 488 Anti-Eg5 antibody [mAbcam 51976] (ab205834)

View more associated products

Overview

  • Product name

    Anti-Eg5 antibody [mAbcam 51976]
    See all Eg5 primary antibodies
  • Description

    Mouse monoclonal [mAbcam 51976] to Eg5
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Eg5 aa 950-1050 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab38094)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HEK293, Jurkat and A431.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 51976
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin-like
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Centromere

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Eg5 antibody [mAbcam 51976] (ab202571)
    • HRP Anti-Eg5 antibody [mAbcam 51976] (ab202575)
    • Alexa Fluor® 488 Anti-Eg5 antibody [mAbcam 51976] (ab205834)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Eg5 protein (ab152491)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
Use a concentration of 1 µg/ml.
WB (1)
Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
IP
Use at an assay dependent concentration.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
  • Involvement in disease

    Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
  • Sequence similarities

    Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications

    Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Target information above from: UniProt accession P52732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3832 Human
    • Omim: 148760 Human
    • SwissProt: P52732 Human
    • Unigene: 8878 Human
    • Alternative names

      • EG5 antibody
      • HKSP antibody
      • KIF11 antibody
      • KIF11_HUMAN antibody
      • Kinesin family member 11 antibody
      • Kinesin like protein 1 antibody
      • Kinesin-like protein 1 antibody
      • Kinesin-like protein KIF11 antibody
      • Kinesin-like spindle protein HKSP antibody
      • Kinesin-related motor protein Eg5 antibody
      • KNSL1 antibody
      • MCLMR antibody
      • Thyroid receptor-interacting protein 5 antibody
      • TR-interacting protein 5 antibody
      • TRIP-5 antibody
      • TRIP5 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
      Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
      ICC/IF image of ab51976 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab51976, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • Western blot - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
      Western blot - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
      All lanes : Anti-Eg5 antibody [mAbcam 51976] (ab51976) at 5 µg/ml

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution

      Performed under reducing conditions.

      Predicted band size: 120 kDa
      Observed band size: 120 kDa

    • Immunoprecipitation - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
      Immunoprecipitation - Anti-Eg5 antibody [mAbcam 51976] (ab51976)
      Eg5 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Mouse monoclonal to Eg5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab51976.
      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
      Band: 120kDa: Eg5

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (6)

    Publishing research using ab51976? Please let us know so that we can cite the reference in this datasheet.

    ab51976 has been referenced in 6 publications.

    • Wu W  et al. S-trityl-L-cysteine, a novel Eg5 inhibitor, is a potent chemotherapeutic strategy in neuroblastoma. Oncol Lett 16:1023-1030 (2018). PubMed: 29963178
    • Swarnkar S  et al. Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms. Sci Rep 8:17419 (2018). PubMed: 30479371
    • Chien JY  et al. aTAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov 2:16006 (2016). PubMed: 27551500
    • Akera T  et al. Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore. Nat Cell Biol 17:1124-33 (2015). PubMed: 26258632
    • Drosopoulos K  et al. APC/C is an essential regulator of centrosome clustering. Nat Commun 5:3686 (2014). PubMed: 24751481
    • Dai J  et al. Studies of haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion. J Cell Sci 122:4168-76 (2009). ICC/IF ; Human . PubMed: 19910498

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Eg5 antibody [mAbcam 51976]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Xenopus laevis Cell (Spindles assembled in Xenopus laevis cytoplasmic e)
    Specification
    Spindles assembled in Xenopus laevis cytoplasmic e
    Fixative
    Formaldehyde and Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 17 2011

    Western blot abreview for Anti-Eg5 antibody [mAbcam 51976]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - other (Xenopus laevis and tropicalis cytoplasmic egg extr)
    Loading amount
    40 µg
    Specification
    Xenopus laevis and tropicalis cytoplasmic egg extr
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 17 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Eg5 antibody [mAbcam 51976]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Humman Mammary Epithelial Cells)
    Specification
    Humman Mammary Epithelial Cells
    Fixative
    Methanol
    Permeabilization
    Yes - PBS1x-1%TritonX
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Read More

    Ms. Judit Pampalona-Sala

    Verified customer

    Submitted Aug 13 2008

    Question

    Could we get blocking peptide for ab51976?

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for contacting us.

    We currently do not hold nay stock of this peptide however if ordered the product can be delivered within 4-6 weeks.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 18 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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