Overview

  • Product name

    Anti-Eg5 antibody [mAbcam 51976] (HRP)
    See all Eg5 primary antibodies
  • Description

    Mouse monoclonal [mAbcam 51976] to Eg5 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Eg5 aa 950-1050 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab38094)

  • Positive control

    • WB:HEK293, Jurkat and A431 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab202575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).

Target

  • Function

    Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
  • Involvement in disease

    Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.
  • Sequence similarities

    Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications

    Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt
  • Database links

  • Alternative names

    • EG5 antibody
    • HKSP antibody
    • KIF11 antibody
    • KIF11_HUMAN antibody
    • Kinesin family member 11 antibody
    • Kinesin like protein 1 antibody
    • Kinesin-like protein 1 antibody
    • Kinesin-like protein KIF11 antibody
    • Kinesin-like spindle protein HKSP antibody
    • Kinesin-related motor protein Eg5 antibody
    • KNSL1 antibody
    • MCLMR antibody
    • Thyroid receptor-interacting protein 5 antibody
    • TR-interacting protein 5 antibody
    • TRIP-5 antibody
    • TRIP5 antibody
    see all

Images

  • All lanes : Anti-Eg5 antibody [mAbcam 51976] (HRP) (ab202575) at 1/5000 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202575 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

     

References

ab202575 has not yet been referenced specifically in any publications.

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