Key features and details
- Rabbit polyclonal to EGF
- Suitable for: WB
- Reacts with: Pig
- Isotype: IgG
Product nameAnti-EGF antibody
See all EGF primary antibodies
DescriptionRabbit polyclonal to EGF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Pig
Recombinant fragment (His-tag) corresponding to Pig EGF aa 45-275. (Expressed in E.coli).
APFLIFSHGNSIFRIDLEGTNHEQLVADAGISVLMDFHYNEERIYWVDLE RQLLQRVFLNGTRQEKVCNLEKNVSGMAINWINEELIWSNQQEGTITVTD MKGNNSRVLLSALKYPANVAVDPVERLMFWSSVVAGSLHRADVTGVEVRL LLETSEEIAAVSLDVLDKRLFWIQYNREGGSSRICSCDYDGGSVHFSKHL TQHNVFAMSLFGDHIFYSTWKKKTIWVANKH
Database link: Q00968
- WB: Porcine uterus lysate; Recombinant porcine EGF protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231103 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 134 kDa.|
FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
Involvement in diseaseHypomagnesemia 4
Sequence similaritiesContains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
- Information by UniProt
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231103 has not yet been referenced specifically in any publications.