Overview

  • Product name

  • Description

    Rabbit polyclonal to EGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, Indirect ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant Human EGF

Properties

Applications

Our Abpromise guarantee covers the use of ab94898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/0.5 - 1/2.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 134 kDa. Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10.

Target

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease

    Hypomagnesemia 4
  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta urogastrone antibody
    • beta-urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

References

ab94898 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your answer.

We guarantee the ab9695 for WB under either reducing or non-reducing conditions. The free of charge replacement will also be covered by our Abcam guarantee so if you would experience problems we would be pleased to help in this case as well.

Please let me know about your decision in this case. i look forward hearing from you if you decide to receive the ab9695 as a replacement.

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Answer

Thank you for your email and the great effort to translate everything into English. I am very sorry, I confused the languages in your email and wrote you in German. I hope you will have it easier to read this email.

Thank you very much for confirming the protein sequence. That shows that this antibody is detecting the native protein, as is also your ELISA experiment.

I contacted the lab to get some confirmation if it was observed before that this antibody has problems to detect the denatured protein.

I am apologise for the inconvenience you had perform the dot blot with this antibody and would like to offer you some compensation in this case.

We offer four other antibodies against EGF, which are covered by our guarantee for WB and human as follows.

1. Anti-EGF antibody (ab9695)

2. Anti-EGF antibody [1B11-3E12] (ab40644)

3. Anti-EGF antibody [EGF-2] (ab8282)

4. Anti-EGF antibody (Biotin) (ab84263)

If one of these suits your experiment I would be pleased to send you a free of charge replacement of one of them. If none of this antibodies full fills your needs I would also be pleased to send you a different antibody as replacement or to give you a credit note.

Thank you very much for your cooperation. I look forward hearing from you how you would like to proceed.

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Answer

Vielen Dank für diese zusätzliche Information.

Können Sie mir bitte bestätigen, ob Sie für den sELISA auch den ab94898 verwenden? Das würde darauf hindeuten, dass dieser das EGF im denaturierten Zustand scheinbar schlecht detektieren kann.

Wenn der gleichen Antikörper verwendet wurde, würde das außerdem darauf hindeuten, dass das verwendete Immunogen für diesen Antikörper mit der Sequenz Ihres rekombinanten Proteins übereinstimmt.

Wenn ein anderer Antikörper verwendet wurde wäre es hilfreich, wenn Sie die Proteinsequenz des recombinanten EGF bei enzo life sciences erfragen könnten, um diese mit dem Immungen des ab94898 abzugleichen.

Vielen Dank im Voraus für Ihre Kooperation. Ich freue mich auf Ihre Antwort.

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I would like to reassure you that this antibody is tested and covered by our guarantee for WB (dotBlot) and human samples. In case a product is not working as stated on the datasheet it will be covered by our six month guarantee.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the provided information, I would ask you to confirm a few more details.

1. Could you please confirm the order number and date of purchase.

2. Can you confirm the sequence of the recombinant hEGF. Is this a full length recombinant protein or a fragment and if it contains a tag?

Thank you for your cooperation. I look forward to hearing from you with the requested details.

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Answer

Thank you for your enquiry.

I can confirm that this particular antibody is sourced externally. The information we have is the following:

'To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.'

Normally the antibody should also recognize the larger precursor form. For immunization the native EGF protein was used which is part of the precursor.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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