- Datasheet
- Specific References (11)
- Protocols
Overview
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Product nameAnti-EGF antibody
See all EGF primary antibodies -
DescriptionRabbit polyclonal to EGF
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, Neutralising, Sandwich ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Highly pure (>98%) recombinant hEGF (human Epidermal Growth Factor)
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Positive control
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa. | |
| ELISA | Use a concentration of 0.5 µg/ml. To detect hEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF. | |
| Neutralising | Use a concentration of 0.25 - 0.35 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 µg/ml of this antibody is required. | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab18635 as Capture. |
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| IHC-P | Use a concentration of 1 µg/ml. |
Target
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FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
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Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
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Involvement in diseaseHypomagnesemia 4
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Sequence similaritiesContains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
Post-translational
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 1950 Human
- Entrez Gene: 13645 Mouse
- Omim: 131530 Human
- SwissProt: P01133 Human
- SwissProt: P01132 Mouse
- Unigene: 419815 Human
- Unigene: 252481 Mouse
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Alternative names
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody (ab9695)ab9695 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. -
Western blot - Anti-EGF antibody (ab9695)Primary ab9695 was used at a dilution of 0.2 ug/ml.
Lane 1: 250 ng Recombinant human EGF protein
Lane 2: 125 ng Recombinant human EGF protein
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody (ab9695)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of murine forepaw vessel, staining EGF with ab9695.
Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (4 µg/ml in BSA in TBS) for 1 hour. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody. -
Sandwich ELISA - Anti-EGF antibody (ab9695)Standard Curve for EGF (Analyte: ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml
References
This product has been referenced in:
- Zhang B et al. Metastasis-associated protein 2 promotes the metastasis of non-small cell lung carcinoma by regulating the ERK/AKT and VEGF signaling pathways. Mol Med Rep 17:4899-4908 (2018). Read more (PubMed: 29393472) »
- Fang C et al. Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway. Exp Ther Med 15:3530-3536 (2018). Read more (PubMed: 29545879) »
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