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    egf-antibody-ab9695.pdf

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Signal Transduction Growth Factors/Hormones EGF
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Anti-EGF antibody (ab9695)

  • Datasheet
  • SDS
Reviews (3)Q&A (7)References (28)

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Western blot - Anti-EGF antibody (ab9695)
  • Sandwich ELISA - Anti-EGF antibody (ab9695)

Key features and details

  • Rabbit polyclonal to EGF
  • Suitable for: WB, IHC-P, ELISA, Neutralising, Sandwich ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-EGF antibody [EPR19899] (ab206423)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-EGF antibody
    See all EGF primary antibodies
  • Description

    Rabbit polyclonal to EGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ELISA, Neutralising, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant full length protein corresponding to Human EGF. Highly pure (>98%) recombinant hEGF (human Epidermal Growth Factor)
    Database link: P01133

  • Positive control

    • Purchase matching WB positive control:Recombinant human EGF protein (Animal Free)
  • General notes

    Although some customers have been successful in IHC we no longer batch test in this application. For IHC validate antibody please see ab184265.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200µl of sterile water.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Light chain type

    unknown
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Growth factors
    • EGF
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Neuroscience
    • Processes

Associated products

  • Positive Controls

    • Recombinant human EGF protein (Animal Free) (ab9697)
  • Recombinant Protein

    • Recombinant human EGF protein (Active) (ab259398)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 0.1 - 0.2 µg/ml. To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa.
IHC-P (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

To detect hEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF.

Neutralising
Use at an assay dependent concentration.

To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 µg/ml of this antibody is required.

Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab18635 as Capture.

Notes
WB
Use a concentration of 0.1 - 0.2 µg/ml. To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

To detect hEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF.

Neutralising
Use at an assay dependent concentration.

To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 µg/ml of this antibody is required.

Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab18635 as Capture.

Target

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease

    Hypomagnesemia 4
  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P01133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1950 Human
    • Omim: 131530 Human
    • SwissProt: P01133 Human
    • Unigene: 419815 Human
    • Alternative names

      • Beta urogastrone antibody
      • beta-urogastrone antibody
      • EGF antibody
      • EGF_HUMAN antibody
      • Epidermal growth factor antibody
      • HOMG4 antibody
      • OTTHUMP00000219721 antibody
      • OTTHUMP00000219722 antibody
      • Pro epidermal growth factor antibody
      • URG antibody
      • Urogastrone antibody
      see all

    Images

    • Western blot - Anti-EGF antibody (ab9695)
      Western blot - Anti-EGF antibody (ab9695)

      Primary ab9695 was used at a dilution of 0.2 ug/ml.

      Lane 1: 250 ng Recombinant human EGF protein

      Lane 2: 125 ng Recombinant human EGF protein

    • Sandwich ELISA - Anti-EGF antibody (ab9695)
      Sandwich ELISA - Anti-EGF antibody (ab9695)

      Standard Curve for EGF (Analyte: ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (28)

    Publishing research using ab9695? Please let us know so that we can cite the reference in this datasheet.

    ab9695 has been referenced in 28 publications.

    • Ma M  et al. SMAD-specific E3 ubiquitin ligase 2 promotes angiogenesis by facilitating PTX3 degradation in MSCs from patients with ankylosing spondylitis. Stem Cells 39:581-599 (2021). PubMed: 33547700
    • Wu M  et al. P25 Gene Knockout Contributes to Human Epidermal Growth Factor Production in Transgenic Silkworms. Int J Mol Sci 22:N/A (2021). PubMed: 33800168
    • Mao Y  et al. The Overexpression of Epidermal Growth Factor (EGF) in HaCaT Cells Promotes the Proliferation, Migration, Invasion and Transdifferentiation to Epidermal Stem Cell Immunophenotyping of Adipose-Derived Stem Cells (ADSCs). Int J Stem Cells 13:93-103 (2020). PubMed: 32114740
    • Praktiknjo SD  et al. Tracing tumorigenesis in a solid tumor model at single-cell resolution. Nat Commun 11:991 (2020). PubMed: 32080185
    • Haque ASMR  et al. CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production. Sci Rep 9:14611 (2019). PubMed: 31601953
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EGF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Forepaw Vessel)
    Specification
    Forepaw Vessel
    Fixative
    Formaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Prot K
    Permeabilization
    No
    Blocking step
    DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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