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mouse tumor tissue (from lung) with human cell line injected, some muscle tissue attached as well
sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF)
Ab: 1 ug/ml 3h RT
block: horse serum + chicken egg white albumin
AR: none + proteinase K
suggested: try no primary crtl to determine if problem is secondary
Asked on May 15 2012
Thank you for contacting us.
I am sorry to hear that the antibodies are not working as expected.
As we discussed over the phone, if you please could try the no primary control, this would help us a lot in determining whether the problem comes from the secondary antibody alone, or if there are issues with the primary antibody and isotype control as well.
Please let me know your results.
I look forward to hear back from you and assist you further in resolving his problem.
Answered on May 15 2012