Key features and details
- Rabbit polyclonal to EGF (Biotin)
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-EGF antibody (Biotin)
See all EGF primary antibodies
DescriptionRabbit polyclonal to EGF (Biotin)
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant human EGF
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Purification notesab84263 is affinity purified and sterile filtered.
Our Abpromise guarantee covers the use of ab84263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 134 kDa.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
Involvement in diseaseHypomagnesemia 4
Sequence similaritiesContains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
- Information by UniProt
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84263 has not yet been referenced specifically in any publications.