• Product name
    Anti-EGF antibody [EGF-10]
    See all EGF primary antibodies
  • Description
    Mouse monoclonal [EGF-10] to EGF
  • Host species
  • Tested applications
    Suitable for: Dot blot, IHC-Fr, ELISA, RIA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant full length protein (Human).

  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.



Our Abpromise guarantee covers the use of ab10409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use a concentration of 0.1 µg/ml.
IHC-Fr 1/500. PubMed: 17475821(see Abreview)
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P 1/500.


  • Function
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity
    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease
    Hypomagnesemia 4
  • Sequence similarities
    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta urogastrone antibody
    • beta-urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all


  • ab10409 staining EGF in human normal skin tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde. Samples were blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/500 dilution for 30 minutes at 21°C. An undiluted HRP-conjugated goat polyclonal was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Lindenmeyer MT  et al. Interstitial vascular rarefaction and reduced VEGF-A expression in human diabetic nephropathy. J Am Soc Nephrol 18:1765-76 (2007). IHC-P, IHC-Fr ; Human . Read more (PubMed: 17475821) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More

Thank you for contacting Abcam regarding ab10409. I have confirmed the following protocol was used in IHC-P: Heat Induced Epitope Retrieval (HIER) is performed by heating the slides immersed in retrieval solution: 10 mM sodium citrate buffer, p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (Normal Skin)
Normal Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 11 2011

Thank you for your enquiry. We tested ab10409 with a recombinant EGF sample, which is available from Sigma I believe, catalogue number E9644. Please do not hesitate to contact me again if you require further information,

Thanks for your enquiry regarding ab10409. Unfortunately we do not currently offer free trial size batches of antibodies to test. I should take this opportunity to mention that this antibody may not recognise rat EGF since the antibody is known not...

Read More

Thank you for your note. We are very sorry to hear that you are still struggling with this antibody and even the replacement vial does not perform as it should do. We can offer you either a credit note or a full refund; please do let us know how to...

Read More

Thank you for your enquiry and forwarding your detailed protocol to us. We are very sorry to hear that you are having problem with this antibody. We would suggest loading purified human EGF (1-100 ng) as positive control along with the samples. ...

Read More

We are not aware of any publications where this antibody is used. However, if you have a look at the reviews section of the online datasheet, you'll see that another Abcam customer used this antibody in IHC on formalin-fixed paraffin-embedded human aut...

Read More


Sign up