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RecombinantRabMAb

Recombinant Anti-EGF antibody [EPR19173] (ab184265)

Submit a review Submit a question References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
  • Western blot - Anti-EGF antibody [EPR19173] (ab184265)
  • Western blot - Anti-EGF antibody [EPR19173] (ab184265)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
  • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] (ab184265)
  • Anti-EGF antibody [EPR19173] (ab184265)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19173] to EGF
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-EGF antibody [EPR19173]
    See all EGF primary antibodies

  • Description

    Rabbit monoclonal [EPR19173] to EGF

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat kidney lysates; Mouse urine lysate. IHC-P: Mouse and rat kidney tissues. IHC-Fr: Mouse kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20)
WB
1/1000. Detects a band of approximately 160, 97 ,66 ,56 ,45 ,27 kDa (predicted molecular weight: 133 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684))
Notes
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20)
WB
1/1000. Detects a band of approximately 160, 97 ,66 ,56 ,45 ,27 kDa (predicted molecular weight: 133 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684))

Target

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.

  • Involvement in disease

    Hypomagnesemia 4

  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.

  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta urogastrone antibody
      • beta-urogastrone antibody
      • EGF antibody
      • EGF_HUMAN antibody
      • Epidermal growth factor antibody
      • HOMG4 antibody
      • OTTHUMP00000219721 antibody
      • OTTHUMP00000219722 antibody
      • Pro epidermal growth factor antibody
      • URG antibody
      • Urogastrone antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of mouse kidney [PMID 1930151]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-EGF antibody [EPR19173] (ab184265)
      Western blot - Anti-EGF antibody [EPR19173] (ab184265)
      All lanes : Anti-EGF antibody [EPR19173] (ab184265) at 1/1000 dilution

      Lane 1 : Mouse kidney lysate
      Lane 2 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 160,45,56,66,97 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.

      The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2260661).

    • Western blot - Anti-EGF antibody [EPR19173] (ab184265)
      Western blot - Anti-EGF antibody [EPR19173] (ab184265)
      Anti-EGF antibody [EPR19173] (ab184265) at 1/5000 dilution + Mouse urine lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 160,27,45,56,66,97 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight  observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2260661).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of rat kidney [PMID 1930151]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)

      Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      No staining on mouse stomach.

      Counter stained with Hematoxylin.

       

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] (ab184265)
      Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] (ab184265)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184265 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Positive staining on mouse kidney was observed. 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Anti-EGF antibody [EPR19173] (ab184265)
      Anti-EGF antibody [EPR19173] (ab184265)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (4)

    Publishing research using ab184265? Please let us know so that we can cite the reference in this datasheet.

    ab184265 has been referenced in 4 publications.

    • Chu H  et al. SERPINE2 feedback regulates EGF/EGFR signaling in human papillary thyroid carcinoma cells. Mol Med Rep 23:N/A (2021). PubMed: 33760135
    • Li Y  et al. Effects of a Chlorogenic Acid-Containing Herbal Medicine (LASNB) on Colon Cancer. Evid Based Complement Alternat Med 2021:9923467 (2021). PubMed: 34462643
    • Du F  et al. Forkhead box K2 promotes human colorectal cancer metastasis by upregulating ZEB1 and EGFR. Theranostics 9:3879-3902 (2019). PubMed: 31281520
    • Zhang Y  et al. Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor. Sci Rep 8:5994 (2018). PubMed: 29662125

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