Recombinant Anti-EGF antibody [EPR19173] (ab184265)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-264966-anti-egf-antibody-epr19173-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19173] to EGF
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-EGF antibody [EPR19173]
See all EGF primary antibodies -
Description
Rabbit monoclonal [EPR19173] to EGF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat kidney lysates; Mouse urine lysate. IHC-P: Mouse and rat kidney tissues. IHC-Fr: Mouse kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19173 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/500.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20) |
|
| WB |
1/1000. Detects a band of approximately 160, 97 ,66 ,56 ,45 ,27 kDa (predicted molecular weight: 133 kDa).
|
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)) |
| Notes |
|---|
|
IHC-Fr
1/500. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20) |
|
WB
1/1000. Detects a band of approximately 160, 97 ,66 ,56 ,45 ,27 kDa (predicted molecular weight: 133 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)) |
Target
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Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate. -
Involvement in disease
Hypomagnesemia 4 -
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
Post-translational
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13645 Mouse
- Entrez Gene: 25313 Rat
- SwissProt: P01132 Mouse
- SwissProt: P07522 Rat
- Unigene: 252481 Mouse
- Unigene: 6075 Rat
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Alternative names
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of mouse kidney [PMID 1930151]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-264969-anti-egf-antibody-epr19173-western-blot.jpg)
Western blot - Anti-EGF antibody [EPR19173] (ab184265)All lanes : Anti-EGF antibody [EPR19173] (ab184265) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 160,45,56,66,97 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2260661).
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![Western blot - Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-264967-anti-egf-antibody-epr19173-western-blot.jpg)
Western blot - Anti-EGF antibody [EPR19173] (ab184265)Anti-EGF antibody [EPR19173] (ab184265) at 1/5000 dilution + Mouse urine lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 160,27,45,56,66,97 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2260661).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-264965-anti-egf-antibody-epr19173-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of rat kidney [PMID 1930151]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-264963-anti-egf-antibody-epr19173-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] (ab184265)Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
No staining on mouse stomach.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-264962-anti-egf-antibody-epr19173-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] (ab184265)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184265 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Positive staining on mouse kidney was observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-EGF antibody [EPR19173] (ab184265)](https://www.abcam.com/ps/products/184/ab184265/Images/ab184265-8-benefits-of-recombinant-antibodies.png)
Anti-EGF antibody [EPR19173] (ab184265)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab184265 has been referenced in 2 publications.
- Du F et al. Forkhead box K2 promotes human colorectal cancer metastasis by upregulating ZEB1 and EGFR. Theranostics 9:3879-3902 (2019). PubMed: 31281520
- Zhang Y et al. Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor. Sci Rep 8:5994 (2018). PubMed: 29662125