Recombinant Anti-EGF antibody [EPR19899] (ab206423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19899] to EGF
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-EGF antibody [EPR19899]
See all EGF primary antibodies -
Description
Rabbit monoclonal [EPR19899] to EGF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human EGF recombinant protein (aa971-1023); Mouse EGF recombinant protein (aa977-1029); Mouse salivary gland lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19899 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 6 kDa (predicted molecular weight: 133 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 6 kDa (predicted molecular weight: 133 kDa). |
Target
-
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate. -
Involvement in disease
Hypomagnesemia 4 -
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
Post-translational
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1950 Human
- Entrez Gene: 13645 Mouse
- Omim: 131530 Human
- SwissProt: P01133 Human
- SwissProt: P01132 Mouse
- Unigene: 419815 Human
- Unigene: 252481 Mouse
-
Alternative names
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
Images
-
All lanes : Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution
Lane 1 : Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag
Lane 2 : Recombinant Human EGF recombinant protein (aa971-1023)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDaBlocking and Diluting buffer:5% NFDM/TBST
Exposure time:1 second
-
All lanes : Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution
Lane 1 : Human EGF recombinant protein (aa971-1023)
Lane 2 : Mouse EGF recombinant protein (aa977-1029)
Lysates/proteins at 0.01 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 6 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution + Mouse salivary gland lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 6 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:2268351 & 7499272).
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab206423 has been referenced in 3 publications.
- Zhu C et al. Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery. Cell Death Discov 8:17 (2022). PubMed: 35013115
- He F et al. ATF5 and HIF1a cooperatively activate HIF1 signaling pathway in esophageal cancer. Cell Commun Signal 19:53 (2021). PubMed: 33980247
- Zhou S et al. Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. Cell Res 30:1063-1077 (2020). PubMed: 32839552