Overview

  • Product name

    Anti-EGF antibody [EPR21926]
    See all EGF primary antibodies
  • Description

    Rabbit monoclonal [EPR21926] to EGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Recombinant fragment within Rat EGF aa 950-1050. The exact sequence is proprietary.
    Database link: P07522

  • Positive control

    • WB: Rat EGF active protein (aa974-1026), rat salivary gland tissue lysate and rat urine. IHC-P: Rat kidney tissue. IHC-Fr: Rat kidney tissue. IP: Rat salivary gland tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 133 kDa.
IHC-P 1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/500.

Perform heat-mediated antigen retrieval by sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IP 1/30.

Target

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease

    Hypomagnesemia 4
  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta urogastrone antibody
    • beta-urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling EGF with ab218831 at 1/10,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in distal convoluted tubules of rat kidney (PMID 1930151) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • All lanes : Anti-EGF antibody [EPR21926] (ab218831) at 1/5000 dilution

    Lane 1 : Rat salivary gland tissue lysate
    Lane 2 : Rat urine

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    Exposure: Lane 1: 3 minutes; Lane 2: 75 seconds

    The molecular mass observed is consistent with what has been described in the literature (PMID:2268351).

  • Immunohistochemical analysis of frozen rat kidney tissue labeling EGF with ab218831 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on distal convoluted tubule of rat kidney (PMID: 23143303) is observed. Tissue was fixed in 4% PFA and permeabilized using 0.2% Triton X-100.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • EGF was immunoprecipitated from 0.35 mg of rat salivary gland lysate with ab218831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218831 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Rat salivary gland tissue 10 µg (Input). 

    Lane 2: ab218831 IP in rat salivary gland lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218831 in rat salivary gland lysate.

    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.

  • Anti-EGF antibody [EPR21926] (ab218831) at 1/1000 dilution + Rat EGF active protein (aa974-1026), 20ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    The molecular mass observed is consistent with what has been described in the literature (PMID:2268351).

References

ab218831 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218831.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up