Key features and details
- Goat polyclonal to EGF - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EGF antibody - N-terminal
See all EGF primary antibodies
DescriptionGoat polyclonal to EGF - N-terminal
Specificityab219348 is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cat, Dog, Chimpanzee, Ferret, Monkey, Gorilla, Bat
- Human Prostate lysate; Human skin tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 134 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.
Involvement in diseaseHypomagnesemia 4
Sequence similaritiesContains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
- Information by UniProt
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling EGF with ab219348 at 5 μg/ml dilution.
Anti-EGF antibody - N-terminal (ab219348) at 1 µg/ml + Human Prostate lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 134 kDa
The two bands represent glycosylated forms of the immature protein.
ab219348 has not yet been referenced specifically in any publications.