Overview

  • Product name

    Anti-EGF antibody - N-terminal
    See all EGF primary antibodies
  • Description

    Goat polyclonal to EGF - N-terminal
  • Host species

    Goat
  • Specificity

    ab219348 is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cat, Dog, Chimpanzee, Ferret, Monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to Human EGF aa 106-119 (N terminal). (NP_001954.2; NP_001171601.1; NP_001171602.1)
    Sequence:

    SRQERVCNIEKNVS


    Database link: P01133

  • Positive control

    • Human Prostate lysate; Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab219348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 134 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.
  • Involvement in disease

    Hypomagnesemia 4
  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta urogastrone antibody
    • beta-urogastrone antibody
    • EGF antibody
    • EGF_HUMAN antibody
    • Epidermal growth factor antibody
    • HOMG4 antibody
    • OTTHUMP00000219721 antibody
    • OTTHUMP00000219722 antibody
    • Pro epidermal growth factor antibody
    • URG antibody
    • Urogastrone antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling EGF with ab219348 at 5 μg/ml dilution.

  • Anti-EGF antibody - N-terminal (ab219348) at 1 µg/ml + Human Prostate lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 134 kDa



    The two bands represent glycosylated forms of the immature protein.

References

ab219348 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219348.
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