Overview

  • Product name

  • Description

    Rabbit polyclonal to EGFL6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 30-219 of Human EGFL6 (BC038587).

  • Positive control

    • WB: Human fetal kidney lysate. IHC-P: Human fetal liver tissue. ICC/IF: HEK293 cells.

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
  • Sequence similarities

    Belongs to the nephronectin family.
    Contains 5 EGF-like domains.
    Contains 1 MAM domain.
  • Developmental stage

    Only detected in fetal tissues.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EGF like domain multiple 6 antibody
    • EGF repeat containing protein 6 antibody
    • EGF-like protein 6 antibody
    • EGF-like-domain multiple 6 antibody
    • egfl6 antibody
    • EGFL6_HUMAN antibody
    • epidermal growth factor like protein 6 antibody
    • Epidermal growth factor-like protein 6 antibody
    • MAEG antibody
    • MAM and EGF domain containing antibody
    • MAM and EGF domains containing gene protein antibody
    • MAM and EGF domains-containing gene protein antibody
    • W80 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human fetal liver tissue labelling EGFL6 with ab140079 at 1/100 dilution.
  • Anti-EGFL6 antibody (ab140079) at 1/1000 dilution + Human fetal kidney lysate

    Predicted band size: 61 kDa

  • ICC/IF image of ab140079 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab140079, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Zhang QW  et al. EGFL6 promotes cell proliferation in colorectal cancer via regulation of the WNT/ß-catenin pathway. Mol Carcinog N/A:N/A (2019). Read more (PubMed: 30693973) »
  • Chang CC  et al. Validation of EGFL6 expression as a prognostic marker in patients with lung adenocarcinoma in Taiwan: a retrospective study. BMJ Open 8:e021385 (2018). Read more (PubMed: 29934389) »
See all 3 Publications for this product

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