Recombinant Anti-EGFR antibody [31G7] (ab218383)
![Immunocytochemistry - Anti-EGFR antibody [31G7] (ab218383)](https://www.abcam.com/ps/products/218/ab218383/Images/ab218383-459094-anti-egfr-antibody-31g7-immunocytochemistry-a431-human.jpg "Immunocytochemistry - Anti-EGFR antibody [31G7] (ab218383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [31G7] to EGFR
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EGFR antibody [31G7]
See all EGFR primary antibodies -
Description
Mouse monoclonal [31G7] to EGFR -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human EGFR. (Purified from A431 cells).
Database link: P00533 -
Positive control
- ICC and Flow Cyt: A431 cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 8th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
31G7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/50.
|
|
| Flow Cyt |
1/900.
|
| Notes |
|---|
|
ICC
1/50. |
|
Flow Cyt
1/900. |
Target
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Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
Involvement in disease
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
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Database links
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
- Unigene: 605083 Human
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Alternative names
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
Images
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Immunocytochemistry - Anti-EGFR antibody [31G7] (ab218383)
Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell line) cells labeling EGFR with ab218383 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (ab179593) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at (ab179513) at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Negative control: SW620 (Human colorectal adenocarcinoma epithelial cells) (PMID:19789347)
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![Flow Cytometry - Anti-EGFR antibody [31G7] (ab218383)](https://www.abcam.com/ps/products/218/ab218383/Images/ab218383-459093-anti-egfr-antibody-31g7-flow-cytometry-sw620-a431-human.jpg)
Flow Cytometry - Anti-EGFR antibody [31G7] (ab218383)Flow Cytometry analysis of A431 (human epidermoid carcinoma epithelial cell line) cells (Right image) labeling EGFR with ab218383 at 1/900 dilution. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).
Negative control: SW620 (human colorectal adenocarcinoma epithelial cell line) (Left image)
Gated on viable cells. -
![Anti-EGFR antibody [31G7] (ab218383)](https://www.abcam.com/ps/products/218/ab218383/Images/ab218383-459096-benefits-of-recombinant-antibodies.jpg)
Anti-EGFR antibody [31G7] (ab218383)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab218383 has been referenced in 2 publications.
- Petersen I et al. EGFR immunohistochemistry as biomarker for antibody-based therapy of squamous NSCLC - Experience from the first ring trial of the German Quality Assurance Initiative for Pathology (QuIP®). Pathol Res Pract 213:1530-1535 (2017). PubMed: 29108919
- Kim HW et al. Breed- and age-related differences in canine mammary tumors. Can J Vet Res 80:146-55 (2016). PubMed: 27127342